BT-5103/BT-5205
BT 5103 Bioinformatics and Systems Biology (M.Sc Biotechnology)
BT 5205 Genomics and Bioinformatics (M.Sc Applied Microbiology)
Syllabus
Pairwise & Multiple Sequence Alignment
Pairwise sequence similarity: Alignment score and substitution matrices, Gap penalty, Dot plots, The Needleman-Wunsch algorithm, Global and Local Alignment, Types of homology, Database search for sequence similarity (BLAST algorithm, Significance of a hit). Multiple Sequence Alignment: Utility, Progressive & Iterative algorithms, Scoring schemes, Domain search: Position-specific scoring matrix, Hidden Markov Models, PSI-BLAST, InterproScan.
Phylogenetic Analysis
Phylogenetic analysis: Uses, selection of types of sequences, Distance Based (UPGMA, Fitch-Margoliash, and Neighbour joining algorithms), Maximum Parsimony and Maximum likelihood method, determining the quality of phylogenetic trees
Structural Bioinformatics
Secondary structure prediction, Neural networks, Signal peptide prediction. Tertiary Structure Prediction: Homology modelling, Quality Parameters, PDB file format, SwissPDB viewer. Structure-Based Drug Design: Scoring Functions and Molecular Docking.
